Can Nexazyme be added to Rushton's formula and used in an ultrasonic cleaner? I have a 10L US unit.
I used to do a pre scrub with a discdoc brush but found no upside. What did make a difference was allowing the LPs to sit still in the US for a couple minutes rather than in continuos motion. I doubt any brush, even a record brush, can get down deep into the grooves. No bristles on any commercial scrub brush would be small enough in diameter. Even lint brushes won't get deep into a groove.I also use Rushton's formula. I have added an Enzyme step before US cleaning. I spray the Enzyme solution on the record, or get a fabric brush really wet, and use a lint and a bristle brush to scrub the record for about one minute. I think adding this mechanical scrubbing action to help loosen stuff in the grooves before US cleaning is a good thing. For Enzyme solution, I just switched to using a home made solution that I read about in a post. The solution is very simple: just mix the contents of one Nexazyme with one liter of water. You can get Nexazyme at Pharmacies or on Amazon.
I've recently built USRCM with 80 kHz US clean and 80 kHz US rinse tanks and a heated, filtered drying cube
Not sure where I read this but I thought 80K was more likely to cause cavitation o vinyl,
My understanding is that higher frequencies produce smaller bubbles which implode with less force, therefore are safer. 40kHz is a sort of GP frequency that is high enough to do the job safely but low enough to produce cheaper transducers.Not sure where I read this but I thought 80K was more likely to cause cavitation o vinyl, especially some of the softer formulations.
As to using US running @ 80 I thought the subject was put to rest regarding damage occurring on records.
The Cyastat SN is just a quat surfactant (Stearamidopropyldimethyl-2-hydroxyethylammonium nitrate). We've discussed many of it's relatives in this thread that you can substitute. I've made hundreds of these PVA masks playing around with many different formulations and can say they just don't produce the results I am after.I'm trying to gather the ingredients for the reg Williamson peel and does anyone know a source for Cyastat SN ?
Elvanol is on chemical store and everything else is amazon just can't find Cyastat SN
I also use Rushton's formula. I have added an Enzyme step before US cleaning. I spray the Enzyme solution on the record, or get a fabric brush really wet, and use a lint and a bristle brush to scrub the record for about one minute. I think adding this mechanical scrubbing action to help loosen stuff in the grooves before US cleaning is a good thing. For Enzyme solution, I just switched to using a home made solution that I read about in a post. The solution is very simple: just mix the contents of one Nexazyme with one liter of water. You can get Nexazyme at Pharmacies or on Amazon.
The Cyastat SN is just a quat surfactant (Stearamidopropyldimethyl-2-hydroxyethylammonium nitrate). We've discussed many of it's relatives in this thread that you can substitute. I've made hundreds of these PVA masks playing around with many different formulations and can say they just don't produce the results I am after.
The Nexazyme protocol was discussed early in this thread, post 61, but the link page was taken down. Anyways, it works well, just be sure to empty and discard the sticky capsule. Also, never let the solution dry on the record surface during incubation (which should be more like 15 min).
Yes, for convenience this works, but it is overkill and you can get by with one capsule powder per 5 liters, particularly when using at elevated temperatures where, as you have found, activity/efficiency is greater. Also, I use in tap water or specific buffer solutions, not distilled/de-ionized as the enzymes require ions for catalysis and stability. I haven't looked for pure liquid sources, but I have used Sporacidin and found the lipase and amylase activities to be very low in that mix. I assume this is because they aren't present in sufficient quantities and the protease (typically papain or pepsin) is quite active in solution and is digesting them. In any event, this is why I choose to stick with powered (dry) enzyme mixes. In the lab, we keep pure enzymes in solution in the fridge or freezer where activity is low and I would recommend only buying liquid enzymatic solutions that are stored this way.I'd like to confirm that the contents of one capsule of Nexazyme in 1 Liter of RODI water (or better) is the correct ratio.In the future, I'd like to find a liquid concentrate of pure enzymes instead of breaking apart capsules. Many enzyme cleaners contain other surfactants, detergents, alcohols, glycol, etc. which I'd like to avoid. Any thoughts on sources?
I've read that enzyme cleaners are most effective at slightly elevated temperatures like 98 Deg F. I'm planning on making a temperature controlled soaking tank as a pre-clean step.
This is an interesting question. The short answer is yes, as some of the same enzymes (e.g. Papain protease) are used in laundry and other household cleaning solutions where they work in a detergent mixture. The long answer involves levels of activity in a situation where pH is not optimized and salts (ions) required for activity and stability are lacking (since tank solutions are typically made with DI water). Furthermore, I would suspect some denaturation of enzymes (killing their activity) in a solution of cavitation bubbles, but I have never looked into this. Maybe give it a try and see what happens?I guess one question is if Nexazyme needs to be applied as a separate step (I actually use a brush to scrub during enzyme treatment), or can Nexazyme simply be added to the ultrasonic tank solution during the general cleaning process.
Yes, for convenience this works, but it is overkill and you can get by with one capsule powder per 5 liters, particularly when using at elevated temperatures where, as you have found, activity/efficiency is greater. Also, I use in tap water or specific buffer solutions, not distilled/de-ionized as the enzymes require ions for catalysis and stability. I haven't looked for pure liquid sources, but I have used Sporacidin and found the lipase and amylase activities to be very low in that mix. I assume this is because they aren't present in sufficient quantities and the protease (typically papain or pepsin) is quite active in solution and is digesting them. In any event, this is why I choose to stick with powered (dry) enzyme mixes. In the lab, we keep pure enzymes in solution in the fridge or freezer where activity is low and I would recommend only buying liquid enzymatic solutions that are stored this way.
This is an interesting question. The short answer is yes, as some of the same enzymes (e.g. Papain protease) are used in laundry and other household cleaning solutions where they work in a detergent mixture. The long answer involves levels of activity in a situation where pH is not optimized and salts (ions) required for activity and stability are lacking (since tank solutions are typically made with DI water). Furthermore, I would suspect some denaturation of enzymes (killing their activity) in a solution of cavitation bubbles, but I have never looked into this. Maybe give it a try and see what happens?
Using distilled water is not a huge deal if you are using an enzyme mix like Nexazyme as this contains a variety of each enzyme type (six different proteases, for example) that have a range of pH optimums and ionic requirements. Some work fine in dH2O, others need a little Mg2+, etc. 100% activity is not essential, but you don't want alkaline pH and no ions around as a good number of the enzymes aren't active under those conditions. I would never use very hard water or highly chlorinated water straight from the tap on records. Always charcoal filter or use bottled.Hello Phantomrebel
Thank you for clarifying ion requirement for enzyme solution. Unfortunately, I have cleaned many records using DI water with Nexazyme, already. Our tap water here is very hard. Do you see a problem using hard water to make enzyme solution? Would there be a problem scrubbing the record while using hard water enzyme solution, due to the minerals and other stuff in the water? Of course, I rinse again with DI water after enzyme application.
Using distilled water is not a huge deal if you are using an enzyme mix like Nexazyme as this contains a variety of each enzyme type (six different proteases, for example) that have a range of pH optimums and ionic requirements. Some work fine in dH2O, others need a little Mg2+, etc. 100% activity is not essential, but you don't want alkaline pH and no ions around as a good number of the enzymes aren't active under those conditions. I would never use very hard water or highly chlorinated water straight from the tap on records. Always charcoal filter or use bottled.