If I can offer my 2 cents worth here, there are two conditions to consider:
1. Operation with no load under quiescent (no signal) conditions: In this scenario, the 225 is absolutely stable, with no tendency towards high or low frequency instability. In fact, the 225 is stable into any type of load (or no load) connected to it.
2. Operation with no load under dynamic (signal present) conditions: This is a very dangerous condition for amplifiers with high internal impedance and no or little NFB. Think guitar amplifier. With this type of amplifier, removing the load under dynamic conditions can allow the signal developed within the OPT to rise to very high (even clipping) levels, which can produce arcing within the output tubes and output transformer. The MC225 however has a very low internal impedance (for a tube amplifier), and employs global NFB along with the Unity Coupled Output Stage, so that there is very little change in output voltage whether the load is connected or not under dynamic conditions. What that means then is this:
If the Mac is playing along delivering a rather typical 5 watts of average power or so and the load is disconnected, no damage or harm will be done to the amplifier. In fact, as long as the power level is such that it is operating below maximum power output at all times, then removing the load won't do any damage. I'm hardly suggesting you do this, but saying that in those instances where it accidentally happens, then no worries. Now, if the amplifier is in a state of being over-driven and the load is removed, then all bets are off. Now the clipping events can cause huge back EMF levels to occur within the output transformer, with no load connected to absorb them. In this scenario then, the output tubes will likely arc, and likely even the best output transformer as well under this condition.
3. Operation partially loaded is the scenario presented when bi-amping with a full range signal presented to the amplifier, and the cross-over appearing at the speaker. If the amplifier is used (for example) to drive the mid and hi drivers, then because of the crossover, the amplifier will be unloaded at low frequencies since the impedance of the crossover will become very high at those frequencies. So, back to #2 again: If the amplifier is being driven within its normal power capability, then no damage will occur. But if the amplifier is driven such that LF signals would drive it into clipping, then potential damage can be done to the amplifier. This is why bi-amping should always be done with an active crossover before the amplifier. This will allow the mid and hi frequency drivers to be connected directly to the output of the amplifier so that it is always loaded, and no damage can occur from the possibility of being unloaded with large LF signals present.
Dave