When a loudspeaker is connected to an amplifier output, the amplifier provides better control of the speaker motion and the speaker has a flatter frequency response if the output impedance of the amplifier is a lot lower than the impedance of the speaker. This is most often specified as the “amplifier damping factor” into 8 ohms, rather than the actual amplifier output impedance. If the amplifier has a damping factor of 250, the output impedance is 8 ohms divided by 250, or 0.032 ohms. With such a low output impedance, the frequency response of the voltage supplied to the speaker is very flat and the amplifier maintains good control over the cone motion at low frequencies.
If a second speaker is placed in series with the first speaker, each speaker is driven by the combined impedance of the amplifier plus the impedance of the other speaker. Instead of a damping factor of 250, it now drops to slightly less than 1, since the new impedance seen by each speaker is 8.032 ohms (the other speaker plus the amplifier output impedance). It gets even lower when speaker cable resistance is added in. The frequency response is affected by any mismatch in the impedance curves of the two speakers.