It depends upon what my results are intended to be, and the materials used. With a properly assembled glue joint, with either mdf or solid wood, mechanical fasteners are not required at all structurally, and I'll only use them if clamping is going to be difficult. If I have to glue a butt joint in a less than optimum fashion, for instance the frames I knocked together for attenuation panels, where one member is end grain, or when working with plywood - especially edges of plywood, I'll clamp things long enough to hold parts in place while I fasten with screws, nail, pneumatic staples...and then remove the clamps. And with edges of plywood I usually use a glue block of some sort.